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  • 20:31, 3 November 2025 Admin talk contribs created page Hemoglobin test for neutral atmosphere (Created page with "Mix with pH 7.4 buffer to make a hemoglobin solution in the flask. Make the unit air-tight and purge it with nitrogen to make it inert. (Will require freeze-pump-thaw method to also purge the buffer solution of dissolved oxygen as that will ruin this indicator) Once everything is inert, introduce a 0.1 M Sodium Dithionite solution via syringe. This will reduce the iron center from Fe3+ to Fe2+. Hemoglobin cannot bind oxygen in its 3+ state but binds it extremely fast...")